Transfection Reagents
Apexbio Technology LLC PPACK Dihydrochloride 142036-63-3 5mg
PPACK Dihydrochloride is a small-molecule inhibitor targeting human -thrombin It is designed to inhibit thrombin activity thereby blocking downstream platelet aggregation and coagulation pathways PPACK Dihydrochloride exerts its biological activity primarily through selective irreversible inhibition of thrombin by covalently occupying the thrombin active-site serine residue and forming a stable tetrahedral intermediate with the catalytic His57 residue Based on these pharmacological properties PPACK Dihydrochloride holds research potential in biomedical studies as an anticoagulant reagent and for investigating thrombin-mediated coagulation and platelet activation mechanisms
Lonza Walkersville Basic Nucleofector Kit for Primary Mammalian Smooth Muscle Cells
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic Nucleofector Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the Nucleofector 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector Programs is tested in combination with the Basic Nucleofector Solution for smooth muscle cells. Benefits:Optimization can be performed within 1 experiment Detailed protocols guiding through the optimization procedure Optional result fine tuning with help of our Scientific Support TeamTransfection efficiencies up to 95%Viabilities up to 96%Applications:Kit suited for smooth muscle cells from different mammalian species and various organs Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Enzo Life Sciences Nupherin™ Transfection Reagent (3 mg)
Nupherin™ dramatically enhances the performance of commercially available cationic lipids to allow high-efficiency lipofection (10-40%) of neuronal, ganglion and other difficult to transfect cells. Efficient lipofection is limited by the ability of transfected DNA to enter the nucleus – a particular obstacle in nondividing or slow dividing cells like neurons. Nupherin, a peptide based reagent that combines a non-classical nuclear localization sequence (M9) with a nucleotide binding domain, helps to transport DNA into the transfected cell's nucleus. Nupherin has been demonstrated to provide high efficiency lipofection of primary rat hippocampal neurons, chicken embryonic retinal ganglian cells, rat pheochromocytoma (PC12) cells and other recalcitrant cells. Fully compatible with cells grown on glass coverslips, in plastic plates, or in tissue-culture flasks. Formulation: Liquid. Sterile solution. Long Term Storage: -80°C.
Eurofins Discoverx PathHunter β-Arrestin-2 Retroparticles, 4 vials x 0.5. (93-1087)
The PathHunter β-Arrestin-2 Retroparticles are MMLV particles designed to transduce cells of interest with β-Arrestin-2 fused to EA. In conjunction with a plasmid expressing a ProLink-tagged GPCR of interest, this allows for these cells to be used for β-Arrestin-2 recruitment assays. This product uses EFC technology. Ligand induced recruitment of β-Arrestin-2 to the activated GPCR leads to complementation and produces EFC signal. These retroparticles ship as 4 vials of cryopreserved retroparticles. For detailed product information, User Manual and datasheet, visit www.discoverx.com/products/virus/arrestin/β-Arrestin-2-retroparticles-93-1087.
Andwin Scientific TRANSFECTION REAGENT + BUFFER
The jetPRIME™ DNA and siRNA Transfection Reagent is made to guarantee high DNA transfection efficiency and excellent gene silencing. This product is very cost-effective as it uses less reagent and DNA and provides more gentle transfections in comparison to other competitors. It can be used with no difficulty and with serum and antibiotics. This can produce 188 reactions in 6-well plates and has a buffer volume of 60 mL. It is sold individually.
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO RISC-FREE CONTROL (5 NMO
siGLO RISC-Free Control (5 nmol)
Sigma Aldrich Fine Chemicals Biosciences Rapid DNA Ligation Kit, 1 kit
The Rapid DNA Ligation Kit covalently joins either sticky-ended or blunt-ended DNA fragments.
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT SET OF 4 TRANSFECTI
Dharmafect Set of 4 transfection reagent
Sigma Aldrich Fine Chemicals Biosciences X tremeGENETM siRNA Transf
Short interfering RNA (siRNA) can be directlyintroduced intocells by transfection. A lipid-based transfection reagent such as X-tremeGENE siRNA Transfection reagent can provide a convenient reliable and efficient vehicle for delivering siRNAs into animal cells enabling the study of cellular and functional consequences of gene knockdown.This innovative reagent forms a complex with siRNA as well as with mixtures of siRNA and plasmid DNA (cotransfection) and efficiently delivers the nucleic acids into animal cells to induce gene silencing. Transfection is achieved in just a few steps mix and incubate diluted transfection reagent with diluted siRNA then add this complex to cells. Because X-tremeGENE siRNA Transfection Reagent functions exceptionally well in the presence or absence of serum and demonstrates low cytotoxicity it can be used without media changes. The product is animal-component free.ContentsSOLUTIONon filtered through 0.2 mum pore size membrane supplied in polypropy
Cell Applications Inc Cryopreserved Epidermal Keratinocytes (HEK), Neonatal
Cryopreserved Epidermal Keratinocytes (HEK), Neonatal
Cell Applications Inc Cytofect skeletal muscle cell transfection kit
Cytofect skeletal muscle cell transfection kit
HORIZON DISCOVERY BIOSCIENCES LIMITED DHARMAFECT 3 TRANSFECTION REAG
NC1250221 DHARMAFECT 3 TRANSFECTION REAG